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KMID : 1041219720140020081
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Journal of Sericultural and Entomological Science
1972 Volume.14 No. 2 p.81 ~ p.92
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Studies on Pathogenicity of Nosema bombycis Naegeli to Parents and Hybrid in the Silkworm (Bombyx mori L.)
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Kim Jong-Soo
Kim Moon-Hyup
Lim Jong-Sung
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Abstract
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Studies on Pathogenicity of Nosema bombycis Naegeli are summarized as follows: 1. The mortality of the parents, Jam 103 and Jam 104, is remarkbly higher than that of the hybrid, Jam 103Jam 104, whereas there is no difference in the mortality between the parents. 3. In the mortality of the pathogen-concentration, it is increased in order of the following concentrations inoculated, 10 , 10 , 10 and 10 /ml. 3. In the mortality of each instar, it is high in order of 5th, 4th, 3rd, and 2nd instar. 4. In the interaction between the mortalities of the varieties and the concentrations, 1) The mortality shows no differences between the parents and the hybrid in the high concentration of 10 /ml. 2) The mortality of the hybrid is lower than that of the parents in the low concentration of 10 /ml, whereas no difference is found between the parents. 3) The interaction appears at the same level in the middle concentration of 10 /ml to the parents and of 10 /ml to the hybrid. 5. It was pointed out that active immunity depends upon the volume of antigen injection, immunizing period, and injection intervals. In this experiment, it is noticed that the optimum volume of injection is above 20ml and D is the best one of the four treatment (A.B.C.D.). 6. The immune sera indicate such a 12,800 high titer in the indirect method can be obtained from the D immunizing method. Silkworm tissues and N. bombycis spores show self-fluorescence, but it is able to distinguish it from the F.I.T.C. by using the U.V. filter. 7. The midgut epithelium is examined to be the first site of the tissues which are penetrated into and multiplied by the inoculation of the pathogen per os.
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